Peroxidase activity of cardiolipin and cytochrome c complex at quasi-stationary approximation for its reaction states.

Анотація

At the present time, the intensive growth of biotechnologies is due to the active implementation of physical research methods in many areas of bioscience and medicine. This significantly expands the possibilities of biochemical methods of analysis and leads to the discovery of new biochemical phenomena. In particular, the experimental detection of peroxidase activity in the cytochrome c (Cyt) and cardiolipin (CL) complexes is based on the use of such physical methods as the chemiluminescence analysis and EPR spectroscopy, which provide biochemists with broad research opportunities both in vivo and in vitro. It should be noted that, the Cyt-CL complexes are the biocomposite systems formed in the cell membranes (CMs) and in their artificial analogues derived from the lipid mono- and bilayers, having a flat or spherical shape. In turn, the CMs of animals and plants belong to one of the widest classes of the natural composite biosystems. So, according to the mosaic model, it has a lipid layer as a supporting base, the surface and inner part of which contains a mosaic of disordered inclusions from various protein molecules (performing the transport, signaling, channel-forming and catalytic functions). Also, the lipid bilayer, which consists of various saturated and unsaturated lipids, is in itself a disordered mosaic of nanoscale fragments that have a liquid crystalline structure and vary in the size and composition. Thus, the studies of the properties and structural features of composite biosystems (natural and artificial) as well as of the various processes and phenomena in them are extremely important for the development of medicine and pharmacology, because such studies stimulate the elaboration and creation of different biocomposite materials with the desired properties.