Fluorescence study of the effect of silver nanoparticles on insulin amyloid fibril formation

Анотація

Silver nanoparticles (AgNPs) have been the most thoroughly investigatednanomaterialsover the past decades due to its high antibacterial, antifungal and catalytic activities. AgNPshave been applied in water purification systems, in the food and clothing industries, in biomedicine, e.g. as drugs against antibiotic resistantbacteria, for DNA/RNA detection via specificprobes, etc. Chemical and optical properties of AgNPs are tunable, depending on size and shape of nanosilver, which are determined by the method of synthesis. In turn, using AgNPs as biological agents desires application of the principles of “green chemistry”, leading to an eco-friendly and cheap fabrication process. Among the nanofactories capable of forming “ecology clean” AgNPs, fungi are better that plants and bacteria due to the easier large-scale production of nanosilver. Despite a huge number of AgNPs have been formed using fungi, further investigations are neededto establish a correlation between the method of synthesis (including type of fungi, concentration of fungi and AgNO3, temperature, purification conditions) and the physico-chemical properties of nanosilver, that is necessary for the functionalization of AgNPs. One of the important and poorly studied area of potential applications of AgNPs is inhibition of amyloid fibrilformation that is the early marker of the development of the severe human disorders, including Alzheimer’s disease, Parkinson disease, systemic amyloidosis, etc.