Bimane and cyanine dyes as scaffolds for multimodal imaging

Abstract

Rapid development of fluorescent dyes for single molecules tracking, both in vitro and in vivo, requires novel and versatile synthesis methods of fluorescent scaffolds that would accommodate subsequent “decoration” steps. In the present work, we describe several important intermediates steps in the synthesis of dyes. Scheme 1 depicts synthesis of Cyanine 3 (Cy3) with two butanoic acid “arms” and an N-hydroxysuccinamide ester (NHSI).